Home 9 Outcomes 9 Publications 9 Detection of Xylella fastidiosa in Host Plants and Insect Vectors by Droplet Digital PCR

Detection of Xylella fastidiosa in Host Plants and Insect Vectors by Droplet Digital PCR

Authors: Nicola Bodino, Vincenzo Cavalieri, Crescenza Dongiovanni, Maria Saponari, and Domenico Bosco
Date Published: 21/03/2023
Via: APS Publications
Keywords: Epidemiology


Spatial-temporal dynamics of spittlebug populations, together with transmission biology, are of major importance to outline the disease epidemiology of Xylella fastidiosa (Xf) subsp. pauca in Apulian olive groves. The spread rate of Xf is mainly influenced by (i) the pathogen colonization of the host plant; (ii) the acquisition of the pathogen by the vector from an infected plant, and its inoculation to healthy plants; (iii) the vector population dynamics and abundance at different spatial scales; (iv) the dispersal of the vector. In this contribution we summarize the recent advances in research on insect vectors’ traits – points (ii), (iii), (iv) – focusing on those most relevant to Xf epidemic in Apulia. Among the vectors’ bioecological traits influencing Xf epidemic on olive trees, we emphasize: natural infectivity and transmission efficiency, phenological timing of both nymphal and adult stage, the role of semi-natural vegetation as vectors’ reservoir in the agroecosystem and landscape, preferential and directional dispersal capabilities. Despite the research on Xf vectors carried out in Europe in the last decade, key uncertainties on insect vectors remain, hampering a thorough understanding of pathogen epidemiology and the development of effective and targeted management strategies. Our goal is to provide a structured and contextualized review of knowledge on Xf vectors’ key traits in Apulian epidemic, highlighting information gaps and stimulating novel research pathways on Xf pathosystems in Europe.

Direct and indirect effects of two endophytic entomopathogenic fungi on survival and feeding behaviour of meadow spittlebug Philaenus spumarius

Authors: Meelad Yousef-Yousef, Marina Morente, Natalia González-Mas, Alberto Fereres, Enrique Quesada-Moraga, Aranzazu Moreno

Date: 01/11/23

Highlights: Direct exposure of Philaenus to Metarhizium brunneum reduces its survivorship. M. brunneum successfully colonises the host plant and reduces survival of Philaenus. Philaenus reaches the xylem faster on M. brunneum-colonised plants. Xylem sap ingestion by...

Functional Peptides for Plant Disease Control

Authors: Emilio Montesinos

Date: 02/06/23

Plant disease control requires novel approaches to mitigate the spread of and losses caused by current, emerging, and re-emerging diseases and to adapt plant protection to global climate change and the restrictions on the use of conventional pesticides. Currently,...

Parallel host shifts in a bacterial plant pathogen suggest independent genetic solutions

Authors: Monica A. Donegan, Helvécio D. Coletta-Filho, Rodrigo P. P. Almeida

Date: 29/03/23

While there are documented host shifts in many bacterial plant pathogens, the genetic foundation of host shifts is largely unknown. Xylella fastidiosa is a bacterial pathogen found in over 600 host plant species. Two parallel host shifts occurred—in Brazil...