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Detection of Xylella fastidiosa in Host Plants and Insect Vectors by Droplet Digital PCR

Authors: Nicola Bodino, Vincenzo Cavalieri, Crescenza Dongiovanni, Maria Saponari, and Domenico Bosco
Date Published: 21/03/2023
Via: APS Publications
Keywords: Epidemiology

Abstract

Spatial-temporal dynamics of spittlebug populations, together with transmission biology, are of major importance to outline the disease epidemiology of Xylella fastidiosa (Xf) subsp. pauca in Apulian olive groves. The spread rate of Xf is mainly influenced by (i) the pathogen colonization of the host plant; (ii) the acquisition of the pathogen by the vector from an infected plant, and its inoculation to healthy plants; (iii) the vector population dynamics and abundance at different spatial scales; (iv) the dispersal of the vector. In this contribution we summarize the recent advances in research on insect vectors’ traits – points (ii), (iii), (iv) – focusing on those most relevant to Xf epidemic in Apulia. Among the vectors’ bioecological traits influencing Xf epidemic on olive trees, we emphasize: natural infectivity and transmission efficiency, phenological timing of both nymphal and adult stage, the role of semi-natural vegetation as vectors’ reservoir in the agroecosystem and landscape, preferential and directional dispersal capabilities. Despite the research on Xf vectors carried out in Europe in the last decade, key uncertainties on insect vectors remain, hampering a thorough understanding of pathogen epidemiology and the development of effective and targeted management strategies. Our goal is to provide a structured and contextualized review of knowledge on Xf vectors’ key traits in Apulian epidemic, highlighting information gaps and stimulating novel research pathways on Xf pathosystems in Europe.

Complete circularized genome resources for Xylella fastidiosa subsp. sandyi strains CO33 and CFBP8478

Authors: María Pilar Velasco-Amo, Luis F. Arias-Giraldo, Miguel Román-Écija, Annalisa Giampetruzzi, Maria Saponari, and Blanca B. Landa

Date: 04/03/24

Xylella fastidiosa (Xf) is a vascular phytopathogenic bacterium native to the Americas, known for causing severe diseases and significant economic losses in important crops. In Europe, Xf is considered a quarantine pathogen. Since its first detection in 2013 in Italy,...

Complete circularized genome resources for Xylella fastidiosa subsp. sandyi strains CO33 and CFBP8478

Authors: María Pilar Velasco-Amo, Luis F. Arias-Giraldo, Miguel Román-Écija, Annalisa Giampetruzzi, Maria Saponari, and Blanca B. Landa

Date: 04/03/24

Xylella fastidiosa (Xf) is a vascular phytopathogenic bacterium native to the Americas, known for causing severe diseases and significant economic losses in important crops. In Europe, Xf is considered a quarantine pathogen. Since its first detection in 2013 in Italy,...

Genealogical tracing of Olea europaea species and pedigree relationships of var. europaea using chloroplast and nuclear markers

Authors: Roberto Mariotti, Angjelina Belaj, Raúl de la Rosa, Rosario Muleo, Marco Cirilli, Ivano Forgione, Maria Cristina Valeri & Soraya Mousavi

Date: 26/09/23

Background Olive is one of the most cultivated species in the Mediterranean Basin and beyond. Despite being extensively studied for its commercial relevance, the origin of cultivated olive and the history of its domestication remain open questions. Here, we present a...