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Complete Circularized Genome Resources of Seven Strains of Xylella fastidiosa subsp. fastidiosa Using Hybrid Assembly Reveals Unknown Plasmids

Authors: María Pilar Velasco-Amo, Luis F. Arias-Giraldo, Miguel Román-Écija, Leonardo De La Fuente, Ester Marco-Noales, Eduardo Moralejo, Juan A. Navas-Cortés, and Blanca B. Landa
Date Published: 01/08/2023
Via: APS
Keywords: Genomic

Abstract

Xylella fastidiosa is a vascular plant pathogenic bacterium native to the Americas that is causing significant epidemics and economic losses in olive and almonds in Europe, where it is a quarantine pathogen. Since its first detection in 2013 in Italy, mandatory surveys across Europe revealed the presence of the bacterium also in France, Spain, and Portugal. Combining Oxford Nanopore Technologies and Illumina sequencing data, we assembled high-quality complete genomes of seven X. fastidiosa subsp. fastidiosa strains isolated from different plants in Spain, the United States, and Mexico. Comparative genomic analyses discovered differences in plasmid content among strains, including plasmids that had been overlooked previously when using the Illumina sequencing platform alone. Interestingly, in strain CFBP8073, intercepted in France from plants imported from Mexico, three plasmids were identified, including two (plasmids pXF-P1.CFBP8073 and pXF-P2.CFBP8073) not previously described in X. fastidiosa and one (pXF5823.CFBP8073) almost identical to a plasmid described in a X. fastidiosa strain from citrus. Plasmids found in the Spanish strains here were similar to those described previously in other strains from the same subspecies and ST1 isolated in the Balearic Islands and the United States. The genome resources from this work will assist in further studies on the role of plasmids in the epidemiology, ecology, and evolution of this plant pathogen.

A high-throughput analysis of high-resolution X-ray CT images of stems of olive and citrus plants resistant and susceptible to Xylella fastidiosa

Authors: Nancy C. Walker, Siul A. Ruiz, Talita R. Ferreira, Helvecio D. Coletta-Filho, James Le Houx, Daniel McKay Fletcher, Steven M. White, Tiina Roose

Date: 27/11/23

The bacterial plant pathogen Xylella fastidiosa causes disease in several globally important crops. However, some cultivars harbour reduced bacterial loads and express few symptoms. Evidence considering plant species in isolation suggests xylem structure influences...

Bioecological traits of spittlebugs and their implications on the epidemiology and control of Xylella fastidiosa epidemic in Apulia

Authors: Nicola Bodino, Vincenzo Cavalieri, Crescenza Dongiovanni, Maria Saponari, Domenico Bosco

Date: 03/11/23

Spatial-temporal dynamics of spittlebug populations, together with transmission biology, are of major importance to outline the disease epidemiology of Xylella fastidiosa subsp. pauca in Apulian olive groves. The spread rate of X. fastidiosa is mainly influenced by...

Direct and indirect effects of two endophytic entomopathogenic fungi on survival and feeding behaviour of meadow spittlebug Philaenus spumarius

Authors: Meelad Yousef-Yousef, Marina Morente, Natalia González-Mas, Alberto Fereres, Enrique Quesada-Moraga, Aranzazu Moreno

Date: 01/11/23

Highlights: Direct exposure of Philaenus to Metarhizium brunneum reduces its survivorship. M. brunneum successfully colonises the host plant and reduces survival of Philaenus. Philaenus reaches the xylem faster on M. brunneum-colonised plants. Xylem sap ingestion by...