Home 9 Outcomes 9 Publications 9 Complete Circularized Genome Resources of Seven Strains of Xylella fastidiosa subsp. fastidiosa Using Hybrid Assembly Reveals Unknown Plasmids

Complete Circularized Genome Resources of Seven Strains of Xylella fastidiosa subsp. fastidiosa Using Hybrid Assembly Reveals Unknown Plasmids

Authors: María Pilar Velasco-Amo, Luis F. Arias-Giraldo, Miguel Román-Écija, Leonardo De La Fuente, Ester Marco-Noales, Eduardo Moralejo, Juan A. Navas-Cortés, and Blanca B. Landa
Date Published: 01/08/2023
Via: APS
Keywords: Genomic

Abstract

Xylella fastidiosa is a vascular plant pathogenic bacterium native to the Americas that is causing significant epidemics and economic losses in olive and almonds in Europe, where it is a quarantine pathogen. Since its first detection in 2013 in Italy, mandatory surveys across Europe revealed the presence of the bacterium also in France, Spain, and Portugal. Combining Oxford Nanopore Technologies and Illumina sequencing data, we assembled high-quality complete genomes of seven X. fastidiosa subsp. fastidiosa strains isolated from different plants in Spain, the United States, and Mexico. Comparative genomic analyses discovered differences in plasmid content among strains, including plasmids that had been overlooked previously when using the Illumina sequencing platform alone. Interestingly, in strain CFBP8073, intercepted in France from plants imported from Mexico, three plasmids were identified, including two (plasmids pXF-P1.CFBP8073 and pXF-P2.CFBP8073) not previously described in X. fastidiosa and one (pXF5823.CFBP8073) almost identical to a plasmid described in a X. fastidiosa strain from citrus. Plasmids found in the Spanish strains here were similar to those described previously in other strains from the same subspecies and ST1 isolated in the Balearic Islands and the United States. The genome resources from this work will assist in further studies on the role of plasmids in the epidemiology, ecology, and evolution of this plant pathogen.

Complete circularized genome resources for Xylella fastidiosa subsp. sandyi strains CO33 and CFBP8478

Authors: María Pilar Velasco-Amo, Luis F. Arias-Giraldo, Miguel Román-Écija, Annalisa Giampetruzzi, Maria Saponari, and Blanca B. Landa

Date: 04/03/24

Xylella fastidiosa (Xf) is a vascular phytopathogenic bacterium native to the Americas, known for causing severe diseases and significant economic losses in important crops. In Europe, Xf is considered a quarantine pathogen. Since its first detection in 2013 in Italy,...

Complete circularized genome resources for Xylella fastidiosa subsp. sandyi strains CO33 and CFBP8478

Authors: María Pilar Velasco-Amo, Luis F. Arias-Giraldo, Miguel Román-Écija, Annalisa Giampetruzzi, Maria Saponari, and Blanca B. Landa

Date: 04/03/24

Xylella fastidiosa (Xf) is a vascular phytopathogenic bacterium native to the Americas, known for causing severe diseases and significant economic losses in important crops. In Europe, Xf is considered a quarantine pathogen. Since its first detection in 2013 in Italy,...

Genealogical tracing of Olea europaea species and pedigree relationships of var. europaea using chloroplast and nuclear markers

Authors: Roberto Mariotti, Angjelina Belaj, Raúl de la Rosa, Rosario Muleo, Marco Cirilli, Ivano Forgione, Maria Cristina Valeri & Soraya Mousavi

Date: 26/09/23

Background Olive is one of the most cultivated species in the Mediterranean Basin and beyond. Despite being extensively studied for its commercial relevance, the origin of cultivated olive and the history of its domestication remain open questions. Here, we present a...