Home 9 Outcomes 9 Publications 9 Complete circularized genome resources for Xylella fastidiosa subsp. sandyi strains CO33 and CFBP8478

Complete circularized genome resources for Xylella fastidiosa subsp. sandyi strains CO33 and CFBP8478

Authors: María Pilar Velasco-Amo, Luis F. Arias-Giraldo, Miguel Román-Écija, Annalisa Giampetruzzi, Maria Saponari, and Blanca B. Landa
Date Published: 04/03/2024
Keywords: Epidemiology | Genomic
DOI: https://doi.org/10.1094/PHYTOFR-01-24-0004-A
Repository link: http://hdl.handle.net/10261/309493

Abstract

Xylella fastidiosa (Xf) is a vascular phytopathogenic bacterium native to the Americas, known for causing severe diseases and significant economic losses in important crops. In Europe, Xf is considered a quarantine pathogen. Since its first detection in 2013 in Italy, mandatory surveys to search for Xf across Europe revealed the presence of three Xf subspecies (fastidiosa, multiplex and pauca) in different outbreaks occurring in France, Spain, and Portugal. Xf subspecies sandyi and morus have not been associated to epidemic outbreaks in Europe; however, subspecies sandyi has been detected in intercepted plants imported from Costa Rica and Honduras. By combining data from the Oxford Nanopore Technologies and Illumina sequencing platforms, we obtained complete genomes for Xf strains CO33 and CFBP8478. Both strains belong to the subspecies sandyi and were isolated from coffee plants intercepted in Europe that were imported from Costa Rica. These two genome resources increase the scarce number of Xf genomes available belonging to the subspecies sandyi which is crucial for comparative genomic analysis and population studies of this important plant pathogenic bacterium.

Modeling the accuracy of Xylella fastidiosa molecular diagnostic tests in naturally-infected almond tree samples

Authors: María Del Pilar Velasco Amo, Concepción Olivares-García, Miguel Román-Écija, Ester Marco-Noales, Juan A. Navas-Cortés, and Blanca Beatriz Landa del Castillo

Date: 19/02/25

Xylella fastidiosa (Xf) is a quarantine plant pathogen in the European Union, recognized as a high-priority pest due to its devastating cultural and economic impact on crops, ornamental plants, and landscape vegetation. The development and implementation of reliable,...

Mitigation of Almond Leaf Scorch by a Peptide that Inhibits the Motility of Xylella fastidiosa

Authors: Luis Moll, Esther Badosa, Leonardo De La Fuente, Emilio Montesinos, Marta Planas, Anna Bonaterra, and Lidia Feliu

Date: 27/01/25

Xylella fastidiosa is a xylem-limited plant pathogenic bacterium that is a menace to the agriculture worldwide, threating economically relevant crops such as almond. The pathogen presents a dual lifestyle in the plant xylem, consisting of sessile microbial aggregates...

Naked-Eye Molecular Testing for the Detection of Xylella fastidiosa in Mallorca (Balearic Island) Almond Orchards by Colorimetric LAMP

Authors: Serena, Amoia Serafina; Falcón-Piñeiro, Ana; Pastar, Milica; Garcìa-Madero, José Manuel; Contaldo, Nicoletta; Muegge, Mikael; Compant, Stéphane; Saldarelli, Pasquale; Minafra, Angelantonio

Date: 13/01/25

Xylella fastidiosa (Xf) is a quarantine pathogen heavily affecting economically important crops worldwide. Different sequence types (STs) belonging to Xf subspecies are present in various areas of Spain, including the Balearic Islands, and cause the...