Home 9 Outcomes 9 Publications 9 Complete Circularized Genome Resources of Seven Strains of Xylella fastidiosa subsp. fastidiosa Using Hybrid Assembly Reveals Unknown Plasmids

Complete Circularized Genome Resources of Seven Strains of Xylella fastidiosa subsp. fastidiosa Using Hybrid Assembly Reveals Unknown Plasmids

Authors: María Pilar Velasco-Amo, Luis F. Arias-Giraldo, Miguel Román-Écija, Leonardo De La Fuente, Ester Marco-Noales, Eduardo Moralejo, Juan A. Navas-Cortés, and Blanca B. Landa
Date Published: 01/08/2023
Via: APS
Keywords: Genomic


Xylella fastidiosa is a vascular plant pathogenic bacterium native to the Americas that is causing significant epidemics and economic losses in olive and almonds in Europe, where it is a quarantine pathogen. Since its first detection in 2013 in Italy, mandatory surveys across Europe revealed the presence of the bacterium also in France, Spain, and Portugal. Combining Oxford Nanopore Technologies and Illumina sequencing data, we assembled high-quality complete genomes of seven X. fastidiosa subsp. fastidiosa strains isolated from different plants in Spain, the United States, and Mexico. Comparative genomic analyses discovered differences in plasmid content among strains, including plasmids that had been overlooked previously when using the Illumina sequencing platform alone. Interestingly, in strain CFBP8073, intercepted in France from plants imported from Mexico, three plasmids were identified, including two (plasmids pXF-P1.CFBP8073 and pXF-P2.CFBP8073) not previously described in X. fastidiosa and one (pXF5823.CFBP8073) almost identical to a plasmid described in a X. fastidiosa strain from citrus. Plasmids found in the Spanish strains here were similar to those described previously in other strains from the same subspecies and ST1 isolated in the Balearic Islands and the United States. The genome resources from this work will assist in further studies on the role of plasmids in the epidemiology, ecology, and evolution of this plant pathogen.

Direct and indirect effects of two endophytic entomopathogenic fungi on survival and feeding behaviour of meadow spittlebug Philaenus spumarius

Authors: Meelad Yousef-Yousef, Marina Morente, Natalia González-Mas, Alberto Fereres, Enrique Quesada-Moraga, Aranzazu Moreno

Date: 01/11/23

Highlights: Direct exposure of Philaenus to Metarhizium brunneum reduces its survivorship. M. brunneum successfully colonises the host plant and reduces survival of Philaenus. Philaenus reaches the xylem faster on M. brunneum-colonised plants. Xylem sap ingestion by...

Functional Peptides for Plant Disease Control

Authors: Emilio Montesinos

Date: 02/06/23

Plant disease control requires novel approaches to mitigate the spread of and losses caused by current, emerging, and re-emerging diseases and to adapt plant protection to global climate change and the restrictions on the use of conventional pesticides. Currently,...

Detection of Xylella fastidiosa in Host Plants and Insect Vectors by Droplet Digital PCR

Authors: Nicola Bodino, Vincenzo Cavalieri, Crescenza Dongiovanni, Maria Saponari, and Domenico Bosco

Date: 21/03/23

Spatial-temporal dynamics of spittlebug populations, together with transmission biology, are of major importance to outline the disease epidemiology of Xylella fastidiosa (Xf) subsp. pauca in Apulian olive groves. The spread rate of Xf is...